Photomultiplier tube calibration Description Automated PMT calibration In flow cytometry, fluorescence intensity is used to 1. 110-004-067. Excite and inspire Instrument start-up, shutdown, and MS or LS separation columns (Miltenyi Biotec, San Diego, CA) 10. Download. The MACSQuant Analyzer 10 brings to life the versatility and power required for modern flow cytometry applications. The MACSQuant X has been engineered from the ground up as a compact flow cytometer made to provide maximum speed and reliability in high-throughput screening. Standard Operating Procedures (SOPs): MACSQuant MACSQuant Calibration beads (Miltenyi Biotec, San Diego, CA) Equipment. Follow this quick guide to see how to easily information refer to section 6.3.5 of the user manual. Miltenyi Biotec GmbH. Optimized flow cytometric protocol for the detection of The same mixed cell suspension (25% GFP+) was used to compare the three cloning methods. Excerpt from MACS&more Vol 14 1/2012 High-sensitivity Nittsu-Eitai Building 5F Contrastes para Equipamentos de Imagem In Vivo de Pequenos Animais Viscover Miltenyi Biotec. Description Multisample processing on the MACSQuant Analyzer is accomplished with the MACS MiniSampler combined with the MACS Reagent Rack 4 and MACS Cooling Tube Racks. The MACSQuant Analyzer 10 templates are available at www.litronlabs.com. Note: If mix sample is selected the sample volume must be entered accurately. User manual - Miltenyi Biotec Keyboard and mouse. Phone: +49 2204 8306-0 . Liquid Chromatography: 10. Page 7 Prerequisite for Single Cell Analysis Manual. Ltd. Unit 16A , 2 Eden Park Drive Macquarie Park NSW 2113 Australia Phone +61 2 8877 7400 Fax +61 2 9889 5044 [email Automacs Magnetic Cell Sorting Separation | Miltenyi MACSQuant Analyzer 10 Instrument specifications 2. Germany . Manual PMT calibration 1. MACSQuant Analyzer 10. MACSQuant Analyzer 10 | June 2018 2/4 Fluidics Minimal uptake volume 1 1 L (25 L recommended for volumetric counting applications) Minimum sample particle size 1 m (recommended) Sample flow rate 25, 50, or 100 L/minute or automated flow rate to maintain 500, 1000, or 2000 events/second Miltenyi Biotec B.V. & Co. KG. Touchscreen. Automatic start, stop and wash function. MACSQuant Instrument quick guide. OctoMACS magnet (Miltenyi Biotec, San Diego, CA) for manual enrichment. Liquid Chromatography: Page 30 No decompression. Maximal care has been taken by Miltenyi Biotec GmbH in the preparation of this user manual. Via Persicetana, 2/D 40012 Calderara di Reno (BO) Italy Phone +39 0516 460 411 Fax +39 0516 460 499 macs@miltenyibiotec.it Japan Miltenyi Biotec K.K. Preview. Friedrich-Ebert-Strae 68 . Thoroughly vortex the MACSQuant Calibration Beads to break up any aggregates. Magnetic enrichment of antigen-specific CD4+ T cells using the MACSQuant Analyzer 10 enables the in-depth characterization of vaccine-induced circulating follicular T helper cells. Bergmann-Leitner, E. et al. (2016) Recently Sold: Miltenyi AutoMACS Subject: Miltenyi AutoMACS Recently Sold This autoMACS Separator is a benchtop instrument for high-speed automated cell sorting. Miltenyi Biotec GmbH . Prime the MACSQuant Instrument and wait a minimum distinguish between positive and negative populations of 30 minutes for warm up of optics. For automated compensation refers to the Miltenyi worksheet manual next to the instrument. Full Cell Viability & Functionnality. Enables re 10 optical channels FSC / SCC, as well as 8 fluorescent channels. 9. 5 L waste bag. Best in class. Our Cytoflex is used for a variety of cells, incl. The MACSQuant Analyzer 10 brings to life the versatility and power required for modern flow cytometry applications. MACSQuant Analyzer integrates magnetic Miltenyi Biotec GmbH, Bergisch Gladbach, Germany manual pre-enrichment, spiked CD326+ cells Miltenyi Biotec MACSQuant Analyzer 10 Cytometer for Sale. 3. Citmetro de Fluxo MACSQuant 10 Miltenyi Biotec. In custom mode, select the Settings tab within the Setting up an experiment on the MACSQuant Analyzer The MACSQuantify Software provides the user a simple interface to program various sample parameters for data acquisition. Prime the MACSQuant Analyzer and wait a minimum of 30 minutes for warm up of optics. 10.1 PropertiesoftheMACSQuantColumn 155 10.2 Example 155 10.3 Pre-enrichmentprograms 158 11 Instrumentmonitoring 159 11.1 Hardwaremonitor 159 11.1.1 Fluidics 159 11.1.2 Sampleuptakeunit 160 11.1.3 Lasersanddetectors 160 11.2 InstrumentstatusLEDs 161 11.3 Remotemonitoring 161 11.3.1 Emailnotification 162 12 MACSQuantLiveSupport 163 MACSQuant Tyto. Automatic calibration. Manual compensation Required materials Single-stained cells or beads with the appropriate fluorochromes for The MACSQuant X has been engineered from the ground up as a compact flow cytometer made to provide maximum speed and reliability in high-throughput screening. It allows you to get more data, in less time, with less effort. Use Miltenyi Biotec offers three different cartridges to perfectly meet your experimental needs. The MACSQuant Tyto Cartridge is your choice to sort fragile cells with a flow rate of up to 55,000 cells/second and 30,000 valve actuations/second. This MACSQuant Instrument user manual applies to the MACSQuant Analyzer. After analysis, we run 5 wells of Trypsin (60 sec, 60uL/min) followed by the Daily Clean Program. 1. 130-097-182. Miltenyi Biotech flow cytometer MACSQuant. Employing the MACS Technology, the autoMACS Separator is designed for positive selection as well as depletion of magnetically labeled cells. CliniMACS Prodigy Tubing Set TS. Miltenyi Biotec contact. Read manual before using the device . of particles. Separador Magntico Manual (Microbeads) de Clulas MACS Miltenyi Biotec. For further information refer to the MACSQuant Instrument user manual and the MACSQuantify Software user guide. 7. 51429 Bergisch Gladbach . For an overview see figure 1. Refer to the MACSQuant Analyzer quick guide: Setting up an experiment for data acquisition in custom mode or the user manual for more details. Whether mining for rare cells, analyzing the efficiency of your cell manufacturing process or investigating signaling pathways, you Sample volume: Enter sample volume. Miltenyi Biotec Inc. 2303 Lindbergh Street, Auburn, CA 95602, USA Phone 800 FOR MACS, +1 530 888 8871, Fax +1 530 888 8925 macs@miltenyibiotec.com page 1/2. MACSQuant Analyzer Instrument specifications Laser 3 air-cooled lasers (excitation wavelengths: 405 nm, 488 nm, and 635 nm) Optical parameters FSC, SSC, and eight fluorescence channels Default detection dyes Violet laser (405 nm): V1: 450/50 nm (VioBlue, Hoechst, Use of the MACSQuant Columns MACSQuant Columns" in the MACSQuant Analyzer user manual for more details. The MACSQuant Tyto is equipped with three lasers, which allows cell sorting with up to 10 parameters. Fax: +49 2204 85197 . Perfect for laboratories that want to optimize time while enjoying the certainty of getting reliable results every single run, the MACSQuant X has been Citmetro de Fluxo MACSQuant 16 Miltenyi Biotec. Ensure single tube holder is correctly attached. Manual cloning by limiting dilution Automated cloning by cellenONE X1 Automated sorting and cloning by Company Website. Page 29 Microchip. Pre-enrichment unit. Miltenyi Biotec MACSQuant Series User Manual. File management Description Experiments An experiment consists of everything that has been In addition to automatically saving data files, the programmed into the Experiment tab, including any MACSQuantify Software enables users to create and save formatting of a Chill Rack, specific annotations, and any various other files 2. .. 500. Employing the MACS Technology, the autoMACS Separator is designed for positive selection as well as depletion of magnetically labeled cells. Best in class. .. Miltenyi Biotec Integrated Workflows. The MACSQuant Tyto is equipped with three lasers (405 nm, 488 nm, 638 nm), two scatter (backscatter, sidescatter) and eight fluorescence channels. Since the actual sorting process completely takes place within the Tyto Cartridge, there is no risk of losing precious sample material and therefore the sorting process is entirely operator-free. For standardized immunofluorescence, staining cells were stained, washed, and analyzed in AutoMACS running buffer (Miltenyi Biotec) using MACSQuant Analyzer10 or 16 (Miltenyi Biotec). MACSQuant VYB (# 130-096-116). Open MACSQuant In Vitro MicroFlow Analysis Template and MACSQuant In Vitro MicroFlow Expt Template with E-mail: [email protected] www.miltenyibiotec.com. 2. At the end of the process enter the desired name for the instrument setting file in the Save window and click the Save button. Recently Sold: Miltenyi AutoMACS Subject: Miltenyi AutoMACS Recently Sold This autoMACS Separator is a benchtop instrument for high-speed automated cell sorting. Description. 1. MACSQuant Instrument quick guide. Whether mining for rare cells, analyzing the efficiency of your cell manufacturing process or investigating signaling pathways, you are equipped for the task at hand. Instrument start-up, shutdown, and basic maintenance MACSQuant Analyzer quick guide Figure 2: Instrument status bar indicating the MACSQuant Analyzer is in data analysis mode (top) or acquisition mode (bottom). Prepare and prime the MACSQuant Analyzer 10. Cell Analytics Facility SOPs MACSQuant Analyzer 21.10.2019 V1 2 Set your gates and the cell number you would like to measure and press Play. Check out our extensive catalog of New & Used Miltenyi Biotec MACSQuant Analyzer 10 Cytometer devices. The easy plug and play format of the instrument and the cartridge, combined with fully automated flow control, laser alignment, and valve timing, makes cell sorting accessible to any lab professional, without intensive training. Figure 1: Priming instrument This version of Miltenyi Biotec MACSQuant 10 Manual compatible with such list of devices, as: MACSQuant 10, MACSQuant 16, MACSQuant Series, MACSQuant VYB Three lasers: blue 488nm, red 635nm, violet 405nm. 2. Compensation Automated or manual with 88 matrix, Channel Laser Filter MACSQuant Analyzer MACSQuant 10 MACSQuant VYB FSC 488 488/10 FSC 561 561/10 SSC 488 488/10 SSC 561 561/10 V1 405 450/50 V2 405 525/50 Miltenyi Biotec MACSQuant Tyto User manual Version 2 EN Original Instructions Miltenyi Biotec GmbH Friedrich-Ebert-Strae 68 51429 Bergisch Gladbach Germany Phone +49 2204 8306-0 Fax +49 2204 85197 macs@miltenyibiotec.de www.miltenyibiotec.com MACSQuant Instruments are compact flow cytometers that combine multisample. 4.3/5.0 (47 reviews) Average rating based on total average product review rating for all company products. At Miltenyi Biotec, were committed to empowering scientific discovery and impacting human lives. MACSQuant Instrument User manual Version 3 EN Original instructions . Advance your flow cytometry: The MACSQuant Analyzer 10The MACSQuant Analyzer 10 is a powerful benchtop flow cytometer for highly sensitive, multi-parameter cell analysis. 23 g syringe needles. Download Operation & users manual of Miltenyi Biotec MACSQuant 10 Measuring Instruments for Free or View it Online on All-Guides.com. For standardized immunofluorescence, staining cells were stained, washed, and analyzed in AutoMACS running buffer (Miltenyi Biotec) using MACSQuant Analyzer10 or 16 (Miltenyi Biotec). It allows you to get more data, in less time, with less effort. The MACSQuant Tyto is a next generation, benchtop cell sorter equipped with 3 lasers, which allows for high speed, 10-parameter cell sorting. MACSQuant Instrument | User manual Version 5 USA/Canada Miltenyi Biotec Inc. 2303 Lindbergh Street Auburn, CA 95602, USA Phone 800 FOR MACS Phone +1 530 888 8871 Fax +1 530 888 8925 [email protected] Australia Miltenyi Biotec Australia Pty. However, Miltenyi Biotec GmbH shall not be liable for any technical or editorial errors or omissions contained herein or for incidental or consequential damages in connection with the furnishing, performance, or use of this document. 10. MACS MiniSampler (96-well plate adapter) Up to 10,000 events per second. 11. 362278045 (# 130-092-197), the MACSQuant Analyzer 10 (# 130-096-343), and the. 700-29. High throughput. More than 200,000 items of Clinical Engineering devices & medical equipment. Miltenyi Biotec GmbH. Miltenyi Biotec SAS 10 rue Mercoeur 75011 Paris, France Phone +33 1 56 98 16 16 Fax +33 1 56 98 16 17 macs@miltenyibiotec.fr Italy Miltenyi Biotec S.r.l. 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